Journal: Microorganisms
Article Title: Screening and Evaluation In Vitro of Bacillus -Based Probiotics for Feed Additives
doi: 10.3390/microorganisms14040834
Figure Lengend Snippet: Heatmap of the strains FJAT-10508 and FJAT-13563 with phylogenetically related Bacillus strains based on the ANI values. Notes: The heatmap analysis was performed using the online tool on the Majorbio Cloud Platform ( https://www.majorbio.com/tools , accessed on 26 December 2025) . The phylogenetically-related Bacillus strains and their genomes included Bacilluus licheniformis ATCC 14580 (GCA_000011645.1), B. pumilus ATCC 7061 (GCA_000172815.1), B. subtilis 168 (GCA 000009045.1), B. thuringiensis ATCC 10792 (GCA_000497525.2), B. velezensis FZB42 (GCA 000015785.2), and Heyndrickxia coagulans DSM 1 (GCA_000832905.1).
Article Snippet: The results showed that the ANI values of the strains FJAT-10508 and FJAT-13563 with the strain B. velezensis FZB42 were higher than 98.35%, exceeding the ANI cut-off value of 96% for species delineation, while those with the strains B. licheniformis ATCC 14580, B. pumilus ATCC 7061, B. subtilis 168, B. thuringiensis ATCC 10792 and Heyndrickxia coagulans (formerly Bacillus coagulans ) DSM 1 were all lower than 88.9% ( ).
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